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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2000/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20 |
| 執筆形態 | その他 |
| 掲載誌名 | CELL |
| 出版社・発行元 | CELL PRESS |
| 巻・号・頁 | 100(5),pp.551-560 |
| 著者・共著者 | Y Abe,T Shodai,T Muto,K Mihara,H Torii,S Nishikawa,T Endo,D Kohda |
| 概要 | Most mitochondrial proteins are synthesized in the cytosol as precursor proteins with a cleavable N-terminal presequence and are imported into mitochondria. We report here the NMR structure of a general import receptor, rat Tom20, in a complex with a presenquence peptide derived from rat aldehyde dehydrogenase, The cytosolic domain of Tom20 forms an all alpha-helical structure with a groove to accommodate the presequence peptide. The bound presequence forms an amphiphilic helical structure with hydrophobic leucines aligned on one side to interact with a hydrophobic patch in the Tom20 groove. Although the positive charges of the presequence are essential for import ability, presequence binding to Tom20 is mediated mainly by hydrophobic rather than ionic interactions. |
| ISSN | 0092-8674 |