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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2003/08 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria |
| 執筆形態 | その他 |
| 掲載誌名 | MOLECULAR BIOLOGY OF THE CELL |
| 出版社・発行元 | AMER SOC CELL BIOLOGY |
| 巻・号・頁 | 14(8),pp.3266-3279 |
| 著者・共著者 | T Yoshihisa,K Yunoki-Esaki,C Ohshima,N Tanaka,T Endo |
| 概要 | Pre-tRNA splicing has been believed to occur in the nucleus. In yeast, the tRNA splicing endonuclease that cleaves the exon-intron junctions of pre-tRNAs consists of Sen54p, Sen2p, Sen34p, and Sen15p and was thought to be an integral membrane protein of the inner nuclear envelope. Here we show that the majority of Sen2p, Sen54p, and the endonuclease activity are not localized in the nucleus, but on the mitochondrial surface. The endonuclease is peripherally associated with the cytosolic surface of the outer mitochondrial membrane. A Sen54p derivative artificially fixed on the mitochondria as an integral membrane protein can functionally replace the authentic Sen54p, whereas mutant proteins defective in mitochondrial localization are not fully active. sen2 mutant cells accumulate unspliced pre-tRNAs in the cytosol under the restrictive conditions, and this export of the pre-tRNAs partly depends on Los1p, yeast exportin-t. It is difficult to explain these results from the view of tRNA splicing in the nucleus. We rather propose a new possibility that tRNA splicing occurs on the mitochondrial surface in yeast. |
| DOI | 10.1091/mbc.e02-11-0757 |
| ISSN | 1059-1524/1939-4586 |
| PMID | 12925762 |