|
若林 憲一 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
|
| 言語種別 | 英語 |
| 発行・発表の年月 | 2021/02 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Thioredoxin pathway in Anabaena sp. PCC 7120: activity of NADPH-thioredoxin reductase C |
| 執筆形態 | その他 |
| 掲載誌名 | The Journal of Biochemistry |
| 掲載区分 | 国外 |
| 出版社・発行元 | Oxford University Press (OUP) |
| 著者・共著者 | Frédéric Deschoenmaeker,Shoko Mihara,Tatsuya Niwa,Hideki Taguchi,Ken-Ichi Wakabayashi,Masakazu Toyoshima,Hiroshi Shimizu,Toru Hisabori |
| 概要 | <title>Abstract</title>
To understand the physiological role of NADPH-thioredoxin reductase C (NTRC) in cyanobacteria, we investigated an NTRC-deficient mutant strain of Anabaena sp., PCC 7120, cultivated under different regimes of nitrogen supplementation and light exposure. The deletion of ntrC did not induce a change in the cell structure and metabolic pathways. However, time-dependent changes in the abundance of specific proteins and metabolites were observed. A decrease in chlorophyll a was correlated with a decrease in chlorophyll a biosynthesis enzymes and photosystem I subunits. The deletion of ntrC led to a deregulation of nitrogen metabolism, including the NtcA accumulation and heterocyst-specific proteins while nitrate ions were available in the culture medium. Interestingly, this deletion resulted in a redox imbalance, indicated by higher peroxide levels, higher catalase activity and the induction of chaperones such as MsrA. Surprisingly, the antioxidant protein 2-CysPrx was downregulated. The deficiency in ntrC also resulted in the accumulation of metabolites such as 6-phosphogluconate, ADP and ATP. Higher levels of NADP+ and NADPH partly correlated with higher G6PDH activity. Rather than impacting protein expression levels, NTRC appears to be involved in the direct regulation of enzymes, especially during the dark-to-light transition period. |
| DOI | 10.1093/jb/mvab014 |
| ISSN | 0021-924X/1756-2651 |
| PMID | 33537746 |
| Put Code(ORCID) | 97421865 |
| PermalinkURL | http://academic.oup.com/jb/advance-article-pdf/doi/10.1093/jb/mvab014/36396993/mvab014.pdf |