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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2007/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Cytoplasmic splicing of tRNA in Saccharomyces cerevisiae |
| 執筆形態 | その他 |
| 掲載誌名 | GENES TO CELLS |
| 出版社・発行元 | WILEY-BLACKWELL |
| 巻・号・頁 | 12(3),pp.285-297 |
| 著者・共著者 | Tohru Yoshihisa,Chie Ohshima,Kaori Yunoki-Esaki,Toshiya Endo |
| 概要 | The splicing of nuclear encoded RNAs, including tRNAs, has been widely believed to occur in the nucleus. However, we recently found that one of the tRNA splicing enzymes, splicing endonuclease, is localized to the outer surface of mitochondria in Saccharomyces cerevisiae. These results suggested the unexpected possibility of tRNA splicing in the cytoplasm. To investigate this possibility, we examined whether cytoplasmic pre-tRNAs are bona fide intermediates for tRNA maturation in vivo. We isolated a new reversible allele of temperature-sensitive (ts) sen2 (HA-sen2-42), which encodes a mutant form of one of the catalytic subunits of yeast splicing endonuclease. The HA-sen2-42 cells accumulated large amounts of pre-tRNAs in the cytoplasm at a restrictive temperature, but the pre-tRNAs were diminished when the cells were transferred to a permissive temperature. Using pulse-chase/hybrid-precipitation techniques, we showed that the pre-tRNAs were not degraded but rather converted into mature tRNAs during incubation at the permissive temperature. These and other results indicate that, in S. cerevisiae, pre-tRNAs in the cytoplasm are genuine substrates for splicing, and that the splicing is indeed carried out in the cytoplasm. |
| DOI | 10.1111/j.1365-2443.2007.01056.x |
| ISSN | 1356-9597/1365-2443 |