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ヨコヤマ ケン
YOKOYAMA KEN
横山 謙 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2018/12/01 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Cryo em structure of intact rotary H+-ATPase/synthase from Thermus thermophilus |
| 執筆形態 | その他 |
| 掲載誌名 | Nature Communications |
| 出版社・発行元 | Nature Publishing Group |
| 巻・号・頁 | 9(1),pp.89 |
| 著者・共著者 | Atsuko Nakanishi,Jun-Ichi Kishikawa,Masatada Tamakoshi,Kaoru Mitsuoka,Ken Yokoyama |
| 概要 | Proton translocating rotary ATPases couple ATP hydrolysis/synthesis, which occurs in the soluble domain, with proton flow through the membrane domain via a rotation of the common central rotor complex against the surrounding peripheral stator apparatus. Here, we present a large data set of single particle cryo-electron micrograph images of the V/A type H+-rotary ATPase from the bacterium Thermus thermophilus, enabling the identification of three rotational states based on the orientation of the rotor subunit. Using masked refinement and classification with signal subtractions, we obtain homogeneous reconstructions for the whole complexes and soluble V1 domains. These reconstructions are of higher resolution than any EM map of intact rotary ATPase reported previously, providing a detailed molecular basis for how the rotary ATPase maintains structural integrity of the peripheral stator apparatus, and confirming the existence of a clear proton translocation path from both sides of the membrane. |
| DOI | 10.1038/s41467-017-02553-6 |
| ISSN | 2041-1723 |
| PMID | 29311594 |